Table 4-1. (Continued)
regression with one specific and one nonspecific component); D: merged analysis (similar to C,
but B is input to find one specific component only); E: merged free receptor plot
b p
(2-parameter simple linear regression performed with nonlinear regression program); F: merged
Hill plot (20%-80% occupancy only, fit by IMSL cubic spline subroutine); G: merged affinity
spectrum (a transform of the log (B-p/Bg) vs. -log F binding plot smoothed by cubic spline);
H: ratio of measured rate constants (described in chapter VI); I: individual Hill plots
(occupancy 0%-80%, simple linear regression).
b. "Asymptotic" s.e.m. generated by SAS nonlinear regression program (Ralston and Jennrich, 1978).
c. Determined from abscissa at 50% occupancy (i.e., logit [Bsp/Bg]=0).
d. Slope at 50% occupancy.
e. Includes 25 single ligand concentration ("saturation") assays.
f. Includes 9 single ligand concentration determinations.